Published June 2012 by CRC Press. 316pp; ISBN: 978-1-43-984825-8
Using a highly accessible style and format, this book:
• Provides an understanding of the underlying principles, benefits, and limitations of optical techniques currently used in cell biology
• Presents a cogent, sophisticated discussion without relying on complex mathematics
• Addresses basic concepts in imaging such as contrasting techniques, fluorescence, and the correction of aberrations
• Explores advanced techniques such as quantitative fluorescence, three-dimensional imaging, nonlinear microscopy, and stimulated emission depletion
• Includes multiple appendices on practical maintenance, cell handling, labeling, and image manipulation
The second edition includes new chapters on super-resolution techniques, and is printed in colour throughought.